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mouse anti golgin84  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti golgin84
    Mouse Anti Golgin84, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/golgin84/anti-Golgin-84/pmc12714868-505-96-101
    Average 94 stars, based on 25 article reviews
    mouse anti golgin84 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Immunolabeling:

    Article Title: Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle fusion and replenishment in a dosage-dependent manner
    Article Snippet: Fixed larvae were mounted in ProLong® Diamond Antifade Mountant (#P36970; Thermo Fisher Scientific, Waltham, MA, USA). .. Antibodies used for immunolabeling were: mouse anti-BRP at 1:500 (Nc82; DSHB), mouse anti-DYN at 1:1000 (Clone 41, Dynamin (RUO); BD Transduction Laboratories, San Jose, CA, USA), mouse anti-Golgin84 at 1:50 (Golgin84 12-1; DSHB), mouse anti-RAB7 at 1:10 (Rab7; DSHB), mouse anti-RFP at 1:1000 (200-301-379; Rockland, Limerick, PA, USA) mouse anti-SYX1 at 1:100 (8C3; DSHB), rabbit anti-CPX at 1:5000 , rabbit anti-NWK at 1:1000 (gift from Avital Rodal), rabbit anti-SYT1 1:500, mouse anti-GFP at 1:1000 (#A-11120; Thermo Fisher Scientific, Waltham, MA, USA), rabbit anti-GFP at 1:1000 (#G10362; Thermo Fisher Scientific, Waltham, MA, USA), mouse anti-RFP at 1:1000 (200-301-379; Rockland), rabbit anti-RFP at 1:1000 (600-401-379; Rockland) and DyLight 649 conjugated anti-HRP at 1:1000 (#123-605-021; Jackson Immuno Research, West Grove, PA, USA). .. Secondary antibodies used for AZ and bouton counting were used at 1:1000: goat anti-rabbit Alexa Flour 488-conjugated antibody (A-11008; Thermofisher) and goat anti-mouse Alexa Fluor 546-conjugated antibody (A-11030; ThermoFisher).

    Enzyme-linked Immunosorbent Assay:

    Article Title: An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules.
    Article Snippet: Cells were then washed 15 min at RT with PBT supplemented with DAPI Jo u rn al o f Ce ll Sc ie n ce (0.2 μg/ml, Sigma D9542), twice for 15 min at RT with PBT and once for 15 min at RT with PBS before mounting in Vectashield medium (Vector H-1000). .. Primary antibodies used were against Calnexin (Cnx99A, DSHB, 1:50), Dcp1 (gift from Elisa Izaurralde, Max Planck Institute for Biology Tübingen, Germany, 1:1000), EEA1 (PCRP-EEA1-1F8, DSHB, 1:50), FLAG (F1804, Sigma, 1:1000), FMR1 (2F5, DSHB, 1:50), GFP (A-11122, Molecular Probes, 1:1000), GMAP (GMAP, DSHB, 1:50), Golgin84 (Golgin84, DSHB, 1:50), HRP-CY3 (123-165-021, Jackson ImmunoResearch, 1:250), Me31B (gift from Akira Nakamura, Kumamoto University, Japan, 1:3000), PABP (gift from Chunghun Lim, Korea Advanced Institute of Science and Technology, Republic of Korea, 1:500), Tral (gift from Akira Nakamura, 1:1000). .. Secondary antibodies used were anti-rabbit-IgG or anti-mouse-IgG conjugated to Alexa Fluor 488, 568 or 647 (Thermo Fisher Scientific, 1:1000).



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